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Isla Holbox is in Quintana Roo, better known worldwide as The Mexican Caribbean
You get to Isla Holbox by taking a ferry in Chiquilá. It's run by the company 9 hermanos, costs $80 pesos and runs daily at 6:00, 8:00, 10:00, 11:00, 12:00, 13:00, 14:00, 16:00, 17:00, 19:00 and 21:30. In weekends also at 15:00 and 18:00. It takes about 25-30 minutes. With two or more people you can charter a fishermans boat for the same price.
To Chiquilá by car: from Cancún (follow signs on the road), take the free highway 180 (called Merida Libre) or Avenida José López Portillo, direction to Merida; keep always on the right also when the road separates. At El Ideal, turn right to Kantunilkin, and then you will arrive to Chiquilá, where the road ends.
There's a few busses daily from Cancún to Chiquilá. From Mérida you can take a nightbus at 23:30, arriving in Chiquilá at 5:30, passing Valladolid around 2:30. If you prefer traveling at daytime, you can take a bus from Mérida or Valladolid to Tizimín and change there to a bus to Chiquilá.
Sunset in Punta Norte, Holbox.
The island of Holbox is in fact very small, you don't see many cars around, so the best way to go anywhere is walking, bicycle or golf carts. You've got a good chance to see flamingo's and lots of other birds if you walk the beach west or east away from town.
- Whale Shark Tours. Tours depart daily from late May through September, with peak season in July and August. Most tours leave at 9AM, returning around 2PM. Requires a one-hour bouncy boat ride but passes dolphins, flamingos and maybe turtles and manta rays. Ceviche lunch included as well as snorkeling at a well-used reef with potentially cloudy visibility. Visitors are generally given three times in the water with the sharks, and no touching is allowed. Standard price is 1200 pesos, although cheaper deals may be found; note that anyone charging less than the standard rate may not be a completely legitimate business and thus may not be following all regulations designed to protect the whale sharks.
- You must try the delicious LOBSTER PIZZA, nowadays an icon of the delicious Holbox gastronomy
- Seafood, delicious in many places for all budgets
- Italian food. Mixed with the delights of the sea and the local preHispanic cuisine
- La serenita (The little mermaid). Very local place, specialized in antojitos (panuchos, sopes, tacos, chalupes, all 10 pesos a piece).
- The Holbox Collection, rooms at various(?) price points
- Casa Sandra  - boutique hotel on the island
- Holbox Dream 
- Hotel La Palapa, Av. Morelos 231, ☎ 0052-984-87-52121, . It offers island-themed rooms and suites, all of which have balcony/deck, Internet connection, and private toilet and bath. Some of its amenities are massage service, restaurant and bar, and recreational activities (whale shark viewing, sunbathing, snorkeling, and windsurfing). Best rates on official website start at USD 69.00.
- Elements  - has a good view from the top
- Maya Inn Holbox  - near to the pier harbor
- Villas Flamingos 
- Wayak,  - rustic and downtown located
- Faro Viejo
- Los Delfines
- Hostal Y Cabanas, Calle Plutarco Elias Calles s/n (At the very end of the fifth street from the pier.), ☎ +529848752358, . Unique, fresh and cozy, the campground is located 80 m from the main beach and offers to backpackers the possibility to sleep on hammocks or beds in small wooden cabañas protected with mosquito nets as well as within tents on the soft sand of the garden. Includes all basic comforts such as hot water, electricity and ventilators. Additional to communal kitchen, laundry, BBQ grill and toilets, the camping provides also a fully equipped house for families and small groups who desire a bit more privacy. Tents from 8 USD per night/person, hammocks from 10 USD per night/person, Beds from 12 USD per night/person & Private cabañas from 45 USD per night..
- Tribu Hostel, Av. Joaquin Coldwell (3 blocks from the pier and 40m from the beach), ☎ +52 984 875 2507, . Brand new hostel with the following facilities: dorm rooms, private rooms, kitchen, internet/wi-fi, book exchange, cinema, bike rental, tours, laundry
- Ida y Vuelta, (near Elements). Nice palapa huts with fan and hammock, sand floors, shared bath (300 pesos), AC rooms (400 pesos). Dorm beds 100 pesos. Shared kitchen. Free wifi. Owners Italian, multi-lingual and can help arrange tours and other activities. 300 pesos.
Creditcards are not widely accepted. Cash is king. There's three ATM's on the island, but they're not very reliable so avoid running out of cash.
Ferry's from Holbox to Chiquilá run at 5:00, 7:00, 9:00, 10:00, 11:00, 12:00, 13:00, 15:00, 16:00, 18:00 and 20:00, and in weekends also on 14:00 and 17:00.
Busses from Chiquila:
- To Cancún: 5:30, 7:30, 13:30. The trip takes 3,5 hours and costs about 83 pesos.
- To Merida: 5:30. The trip takes almost 7 hours and costs 150 pesos.
- To Valladolid: 5:30.
- To Tizimín: 5:30, 7:30, 13:30, 16:30. The trip takes 3 hours and costs about 70 pesos.
Tizimín has two busstations where you'll find busses to several destinations, including Valladolid.
In Valladolid you can catch plenty of busses to Mérida, so you can consider spending some hours here and move on in the evening.
The busses are run by Autobuses del Noreste en Yucatan, are second class and stop at a lot of places during the ride.